Clinical 🅱iochemistry

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🌺 Is the bladder 100% sterile?:
================

🔰The bladder was not originally tested because:
1️⃣ It was considered to be sterile and there were complexities regarding sample collection.
2️⃣ Culture techniques were developed for fast growing aerobic uropathogens.

Over the last couple years it has shown that a urinary microbiome exists and for most individuals it plays a protective role.
◎●━ @Biochem_Lab ━●◎•

🔰It was noticed that patients with UTI symptoms, but negative standard urine cultures, contained slow growing organisms that required different growth conditions than those in standard method.

🔰Later, An enhanced quantitative urine culture (EQUC) protocol was developed.
🔰Unlike the standard urine culture protocol, which plates 1μl urine on blood and MacConkey agar with incubation in air for 24 hours, EQUC plates a greater amount of urine (100μl) on a variety of media with incubation under diverse atmospheric conditions for 48 hours.

The development of EQUC and other enhanced culture protocols showed that
The bladder is not 100% sterile.

https://www.tgoop.com/joinchat-AAAAAECuqoTkgQkHpiHlGA

www.tgoop.com/Biochem_Lab/930

22.2K viewsedited  Jan 4, 2018 at 21:40

✨🍃🍂🌺🍃🍂🌺🍃🍂🌺
🍃🍂🌺🍃🍂🌺
🍂🌺🍂
🌺 Is the bladder 100% sterile?:
================

🔰The bladder was not originally tested because:
1️⃣ It was considered to be sterile and there were complexities regarding sample collection.
2️⃣ Culture techniques were developed for fast growing aerobic uropathogens.

Over the last couple years it has shown that a urinary microbiome exists and for most individuals it plays a protective role.
◎●━ @Biochem_Lab ━●◎•

🔰It was noticed that patients with UTI symptoms, but negative standard urine cultures, contained slow growing organisms that required different growth conditions than those in standard method.

🔰Later, An enhanced quantitative urine culture (EQUC) protocol was developed.
🔰Unlike the standard urine culture protocol, which plates 1μl urine on blood and MacConkey agar with incubation in air for 24 hours, EQUC plates a greater amount of urine (100μl) on a variety of media with incubation under diverse atmospheric conditions for 48 hours.

The development of EQUC and other enhanced culture protocols showed that
The bladder is not 100% sterile.

https://www.tgoop.com/joinchat-AAAAAECuqoTkgQkHpiHlGA

BY Clinical 🅱iochemistry